NEW publications

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T. Tylek, J. Wang, A. Vaughan, K.L. Spiller. “Biomaterial-mediated intracellular control of macrophages for cell therapy in pro-inflammatory and pro-fibrotic conditions.” Biomaterials, 2024
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 L.A. Steele, B. Hernaez-Estrada, K.L. Spiller. “Effects of a bioengineered allogeneic cellularized construct (BACC) on primary human macrophage phenotype.” Advanced Healthcare Materials, 2024

recent publications

G.E. Risser, M. Machour, B. Hernaez-Estrada, D. Li, S. Levenberg, K.L. Spiller. “Effects of Interleukin-4 (IL-4)-Releasing Microparticles and Adoptive Transfer of Macrophages on Immunomodulation and Angiogenesis.” Biomaterials, 2023, 296: 122095.
S. Inamdar, T. Tylek, A.P. Suresh, N.D. Ng, E. Schmitzer, K. Lintecum, Camila de Ávila, John D. Fryer, Ying Xu, K.L. Spiller, A.P. Acharya. “Biomaterials based simultaneous delivery of spermine and alpha ketoglutarate modulate metabolism and innate immune cell phenotype in mice.” Biomaterials, 2023, 293: 121973.
Soni, S., A.M. D’Elia, A. Alsasa, S. Cho, T. Tylek, E.M. O’Brien, R. Whitaker, K.L. Spiller, and C.B. Rodell. “Injectable polymer-nanoparticle hydrogels enable sustained small molecule drug delivery for immune modulation.” Biomaterials Science, 2022, 10: 6951-6967.
​Boerman, O., Z. Abedin, R.A. DiMaria-Ghalili, M.S. Weingarten, P.A. Lewin, K.L. Spiller. “Gene expression changes in therapeutic ultrasound-treated human chronic wound tissue.” Frontiers in Medicine, 2023
Soe, Mi Thant Mon, K.L. Spiller, and Moses Noh. "Dielectrophoretic characterization of macrophage phenotypes." Electrophoresis, 2022, 43: 2440-2452.
Waring, M.S., L.J. Lo, M.A. Kohanski, E. Kahle, I.M. Marcus, H. Smith, K.L. Spiller, S.L. Walker. “Design and quantitative evaluation of ‘Aerosol Biocontainment Device (ABCD)’ for reducing aerosol exposure during infectious aerosol-generating events.” PLoS One, 2022.
Cottler, P.S., H. Kang, V.A. Nash, L. Salopek, A.C. Bruce, K.L. Spiller, C.A. Campbell. “Immunomodulation of acellular dermal matrix through interleukin-4 enhances vascular infiltration.” Annals of Plastic Surgery, 2022, in press. doi: 10.1097/SAP.0000000000003163
Lurier, E.B., V.A. Nash, H.S. Abee, T.B. Wissing, C.V.C. Bouten, A.I.P.M. Smits, K.L. Spiller. “Imparting immunomodulatory to scaffolds by leveraging biotin-avidin interactions.” ACS Biomaterials Science and Engineering, 2021, 7 (12): 5611-5621.
O’Brien, E.M., K.L. Spiller. “Pro-inflammatory polarization primes macrophages to transition into a distinct M2-like phenotype in response to IL-4.” Journal of Leukocyte Biology, 2021, in press. doi.org/10.1002/JLB.3A0520-338R
Hernaez-Estrada, B., A. Gonzalez-Pujana, A. Cuevas, A. Izeta, K.L. Spiller, M. Igartua, E. Santos-Vizcaino, R.M. Hernandez. “Human hair follicle-derived mesenchymal stromal cells from the lower dermal sheath as a competitive alternative for immunomodulation.” Biomedicines, 2022, 10(2): 253
Whitaker, R., B. Hernaez-Estrada, R. Hernandez, E. Santos-Vizcaino, K.L. Spiller. “Immunomodulatory biomaterials for tissue repair.” Chemical Reviews, 2021, 121 (18): 11305-11335.
Eager, J.E., W.J. Warrender, C.B. Deusenbery, G. Jamgochian, A. Singh, J.A. Abboud, K.L. Spiller. “Distinct Gene Expression Profile in Patients With Poor Postoperative Outcomes After Rotator Cuff Repair: A Case-Control Study.” American Journal of Sports Medicine, 2021, 49 (10): 2760-2770
Witherel, C.E., K. Sao, B.K. Brisson, B. Han, S.W. Volk, R.J. Petrie, L. Han, K.L. Spiller. “Regulation of extracellular matrix assembly and structure by hybrid M1/M2 macrophages.” Biomaterials, 2021.
Graney, P.L., S. Ben-Shaul, S. Landau, A. Bajpai, B. Singh, J. Eager, A. Cohen, S. Levenberg, K. L. Spiller. “Macrophages of diverse phenotypes drive vascularization of engineered tissues.” Science Advances, 2020, 6 (18) eaay6391.
Alina, T., V.A. Nash, K.L. Spiller. “Effects of biotin-avidin interactions on hydrogel swelling.” Frontiers in Chemistry, 2020, DOI: 10.3389/fchem.2020.593422
Blatt, S.E., E. B. Lurier, G.E. Risser, K.L. Spiller. “Characterizing the macrophage response to immunomodulatory biomaterials through gene set analyses.” Tissue Engineering Part C: Methods, 2020 
Wofford, K.L., B. Singh, D.K. Cullen, K.L. Spiller. “Biomaterial-mediated Reprogramming of Monocytes via Microparticle Phagocytosis for Sustained Modulation of Macrophage Phenotype.” Acta Biomaterialia, 2019.
Deusenbery, C.B., L. Kalan, J. Meisel, S. Gardner, E. Grice, K.L. Spiller. “Human macrophage response to microbes isolated from diabetic foot ulcers.” Wound Repair and Regeneration, 2019, DOI: 10.1111/wrr.12752
Pentecost, A.E., S. Jeon, Y. Ko, M. Kim, Y. Gogotsi, K. Kim, K.L. Spiller. “Immunomodulatory nanodiamond particles for the treatment of rheumatoid arthritis.” Regenerative Biomaterials. 2019. 6 (3) 163-174.
O’Brien, E.M., G. Risser, K.L. Spiller. “Sequential drug delivery to modulate macrophage behavior and enhance implant integration.” Advanced Drug Delivery Reviews, 2019, DOI: 10.1016/j.addr.2019.05.005
K.L. Wofford, K.C. Cullen, K.L. Spiller. “Modulation of macrophage phenotype via phagocytosis of drug-loaded microparticles.” Journal of Biomedical Materials Research Part A 2019. 
​*Selected for Outstanding PhD Student Award from the Society for Biomaterials.
C.E. Witherel, D. Abebayehu, T.H. Barker, K.L. Spiller. "Macrophage and fibroblast interactions in biomaterial-mediated fibrosis." Advanced Healthcare Materials 2019.
S. Liu+, A. Bajpai+, E.A. Hawthorne, Y. Bae, P. Castagnino, J. Monslow, E. Puré, K.L. Spiller* and Richard K. Assoian*. “Cardiovascular protection in females linked to estrogen-dependent inhibition of arterial stiffening and macrophage MMP12.” JCI Insight 2019.
Paige, J., M. Kremer, J. Landry, S. Hatfield, D. Wathieu, A. Brug, D. Lightfell, K.L. Spiller, T.C. Woods. “Modulation of inflammation in wounds of diabetic patients treated with porcine urinary bladder matrix.” Regenerative Medicine, 2019 (14) 269-277.
Bajpai, A., S. Nadkarni, M. Neidrauer, M.S. Weingarten, P.A. Lewin, K.L. Spiller. “Effects of nonthermal, noncavitational ultrasound exposure on human diabetic ulcer healing and inflammatory gene expression.” Ultrasound in Medicine and Biology, 2018, 44 (9) 2043-2049.
J.R. Alhamdi, T. Peng, I.M. Al-Naggar, K.L. Spiller*, L.T. Kuhn*. “Controlled M1-to-M2 transition of aged macrophages by calcium phosphate coatings.” Biomaterials, 2018, DOI: 10.1016/j.biomaterials.2018.07.012
Karuppagounder, V., A. Bajpai, S. Meng, S. Arumugam, R. Sreedhar, V.V. Giridharan, A. Guha, A. Bhimaraj, K.A. Youker, S.S. Palaniyandi, H. Karmouty-Quintana, K.L. Spiller, K. Watanabe, R.A. Thandavarayan. “Small molecule disruption of G protein beta-gamma subunit signaling reprograms human macrophage phenotype and prevents autoimmune myocarditis in rats.” PLoS One, 2018, 13 (7) e0200697.
Nakazawa, K.R., B.A. Walter, D. Laudier, K.L. Spiller, J.C. Iatridis. “Accumulation and Localization of Macrophage Phenotypes with Human Intervertebral Disc Degeneration." The Spine Journal, 2018.
Pentecost, A.E., C.E. Witherel, Y. Gogotsi, K.L. Spiller. “Anti-inflammatory effects of octadecylamine-functionalized nanodiamond on primary human macrophages.” Biomaterials Science, 2017, DOI: 10.1039/C7BM00294G. 
Witherel, C.E., T. Yu, M. Concannon, W. Dampier, K.L. Spiller. “Immunomodulatory effects of human cryopreserved viable amniotic membrane in a pro-inflammatory environment in vitro.” Cellular and Molecular Bioengineering, 2017.
Spiller, K.L. and T.J. Koh. “Macrophage-based therapeutic strategies in regenerative medicine.” Advanced Drug Delivery Reviews, 2017
Zgheib, C., M.M. Hodges, M.W. Allukian, J. Xu, K.L. Spiller, J.H. Gorman, R.C. Gorman, K.W. Liechty. “Cardiac progenitor cell recruitment drives fetal cardiac regeneration via enhanced angiogenesis.” Annals of Thoracic Surgery, 2017, DOI:10.1016/j.athoracsur.2017.05.040. 
Witherel, C.E., D. Gurevich, J.D. Collin, P. Martin, K.L. Spiller. “Host-biomaterial interactions in zebrafish.” ACS Biomaterial Science and Engineering, 2017.
Ferraro, N.M., W. Dampier, M.S. Weingarten, K.L. Spiller. “Deconvolution of Heterogeneous Wound Tissue Samples into Relative Macrophage Phenotype Composition via Models based on Gene Expression.” Integrative Biology, 2017, DOI: 10.1039/C7IB00018A.
Lurier, E.B., D. Dalton, W. Dampier, S. Nassiri, N.M. Ferraro, P. Raman, R. Rajagopalan, M. Sarmady, K.L. Spiller. “Transcriptome analysis of IL-10-stimulated (M2c) macrophages by next-generation sequencing.” Immunobiology 2017.
​Graney, P.L., E.B. Lurier, K.L. Spiller. “Strategies for the design of biomaterials and bioactive factor delivery systems to control macrophage activation in regenerative medicine.” ACS Biomaterials Science and Engineering, 2017
Wofford, K.L., J.P. Harris, K.D. Browne, D.P. Brown, M.R. Grovola, C.J. Mietus, J.A. Wolf, J.E. Duda, M.E. Putt, K.L. Spiller, D.K. Cullen. “Rapid neuroinflammatory response localized to injured neurons after diffuse traumatic brain injury in swine.” Experimental Neurology 2017 (290) 85-94.
Ersumo, N., C.E. Witherel, K.L. Spiller. “Differences in time-dependent mechanical properties between extruded and molded hydrogels.” Biofabrication 2016.
Zhu, Y., K. Lyapichev, D. Lee, D. Motti, N. Ferraro, S. Yahn, C. Soderblom, J. Zha, J.R. Bethea, K.L. Spiller, V. Lemmon, J. Lee. “Macrophage-specific translational profile reveals lipid catabolic pathways after spinal cord injury.” Journal of Neuroscience 2017
Graney, P.L., I. Roohani-Esfahani, H. Zreiqat, K.L. Spiller. “Response of macrophages to biomaterials used in bone regeneration.” Journal of the Royal Society Interface 2016, DOI: 10.1098/rsif.2016.0346
Garash, R., A. Bajpai, B. Marcinkiewicz, K.L. Spiller. “Drug delivery strategies to control macrophage behavior for tissue regeneration.” Experimental Biology and Medicine 2016, DOI: 10.1177/1535370216649444.
Witherel, C.E., P.L. Graney, D.O. Freytes, M.S. Weingarten, K.L. Spiller. “Response of human macrophages to wound matrices in vitro.” Wound Repair and Regeneration, 2016, DOI: 10.1111/wrr.12423.
Yu, T., W. Wenbo, S. Nassiri, T. Kwan, C. Dang, W. Liu, K.L. Spiller. “Temporal and spatial distribution of macrophage phenotype markers in the foreign body response to glutaraldehyde-crosslinked gelatin hydrogels.” Journal of Biomaterials Science- Polymer Edition, 2016, DOI: 10.1080/09205063.2016.1155881
Hind, L.E., E.B. Lurier, M. Dembo, K.L. Spiller, D.A. Hammer. “Effect of M1-M2 polarization on the motility and traction stresses of primary human macrophages.” Cellular and Molecular Bioengineering 2016, DOI: 10.​1007/​s12195-016-0435-x.
S. Nassiri, I. Zakeri, M.S. Weingarten, K.L. Spiller. "Relative Expression of Pro-Inflammatory and AntiInflammatory Genes Reveals Differences Between Healing and Nonhealing Human Chronic Diabetic Foot Ulcers." Journal of Investigative Dermatology, 2015, DOI: 10.1038/jid.2015.30
Highlighted as "Editor's Choice" in Science Translational Medicine here ​
V. Tutwiler, R. I. Litvinov, A.P. Lozhkin, A.D. Peshkova, T. Lebedeva, F.I. Ataullakhanov, K.L. Spiller, D.B. Cines, J.W. Weisel. “Kinetics and mechanics of clot contraction are governed by molecular and cellular blood composition.” Blood, 2015, DOI: http://dx.doi.org/10.1182/blood-2015-05-647560
T. Yu, V. Tutwiler, K.L. Spiller. "The role of macrophages in the foreign body response to implanted biomaterials." In: Biomaterials and Regenerative Medicine in the Immune System. L. Santambrogio, ed. Springer, 2015.
A. Pentecost, E. Lurier, K.L. Spiller. “Nanoparticulate systems for controlling monocyte/macrophage behavior.” Microscale Technologies for Cell Engineering Applications. Ed: A. Singh, A. Gaharwar. Springer, 2015.
S. Nassiri, P. Graney, K.L. Spiller. "Manipulation of macrophages to enhance bone repair and regeneration." In: A Tissue Regeneration Approach to Bone Repair. H. Zreiqat and V. Rosen, eds. Springer Mechanical Engineering Series, 2014.
K.L. Spiller, E. Wrona, S. Romero-Torres, I. Pallotta, P.L. Graney, C.E. Witherel, L.M. Panicker, R.A. Feldman, A. Urbanksa, L. Santambrogio, G. Vunjak-Novakovic, D.O. Freytes. “Differential Gene Expression in Human, Murine, and Cell Line-derived Macrophages.” Experimental Cell Research, 2015, DOI:10.1016/j.yexcr.2015.10.017.
ARD Reeves, KL Spiller, DO Freytes, G Vunjak-Novakovic, DL Kaplan. 2015. "Controlled release of cytokines using silk-biomaterials for macrophage polarization." Biomaterials 73: 272-283.
K.L. Spiller, S. Nassiri, C. Witherel, R. Anfang, J. Ng, K. Nakazawa, G. Vunjak-Novakovic. 2015. “Sequential delivery of cytokines from bone scaffolds to facilitate the M1-to-M2 transition of macrophages.” Biomaterials 37: 194-207.
K.L. Spiller, D.O. Freytes, G. Vunjak-Novakovic. 2015. "Macrophages modulate engineered human tissues for enhanced vascularization and healing." Annals of Biomedical Engineering 43: 616-627.
K.L. Spiller, R.R. Anfang, K.J. Spiller, J. Ng, K.R. Nakazawa, J.W. Daulton, G. Vunjak-Novakovic. 2014. "The role of macrophage phenotype in vascularization of tissue engineering scaffolds." Biomaterials 35:  4477-4488.
K.L. Spiller, G. Vunjak-Novakovic. 2015. "Clinical translation of controlled protein delivery systems for tissue engineering." Drug Delivery and Translational Research 5(2): 101-115

Science translational medicine editor's choice articles

KL Spiller. 2016. "Pigs in a blanket." Sci Transl Med. 8: 325ec24.
KL Spiller. 2016. "Efficacy by design." Sci Transl Med. 7: 319ec219
KL Spiller. 2015. "Wound healing goes green." Sci Transl Med. 7: 313ec194.
KL Spiller. 2015. "Super Model." Sci Transl Med. 7: 307ec168.
KL Spiller. 2015. "Contagious biomaterials." Sci Transl Med. 7: 301ec143.
K.L. Spiller. 2015. "Battle scars: SAP and CRP." Sci Transl Med. 7:295ec116.
K.L. Spiller. 2015. "Heart attack on a plate." Sci Transl Med. 7: 289ec88.
K.L. Spiller. 2015. "Shaping Up Nicely." Sci Transl Med. 7: 283:ec63.
K.L. Spiller. 2015. "Control under pressure." Sci Transl Med. 7:271ec11.
K.L. Spiller. 2014. "The Clot Thickens..." Sci Transl Med. 6:265ec206.
K.L. Spiller. 2014. "Elbow Grease." Sci Transl Med. 6:259ec180.
K.L. Spiller. 2014. "Culture shock! Brain-like tissue grown in vitro has potential." Sci. Transl. Med. 6:253ec156.
K.L. Spiller. 2014. "How to Build a Better Bone Tumor." Science Transl. Med. 6: 235ec81.
K.L. Spiller. 2014. "Spotlight on Cancer." Science Transl. Med. 6: 229ec54.

other publications

Ng, J., K.L. Spiller, J.C. Bernhard, G. Vunjak-Novakovic. “Biomimetic approaches to bone tissue engineering.” Tissue Engineering Part B: Reviews, 2017
I. Gadjanski, S. Yodmuang, K.L. Spiller, S. Bhumiratana, G. Vunjak-Novakovic. 2013. “Supplementation of exogenous ATP enhances mechanical properties of 3D cell-agarose constructs for cartilage tissue engineering.” Tissue Engineering A 19 (19-20): 2188-2200.

I. Gadjanski, K.L. Spiller, G. Vunjak-Novakovic. 2012. “Time-dependent processes in stem cell-based articular cartilage engineering.” Stem Cells Reviews and Reports 8 (3): 863-881.

K.L. Spiller, Y. Liu, J.L. Holloway, S.A. Maher, W. Liu, Y.L. Cao, G. Zhou, A.M. Lowman.  2012. “A novel method for the direct fabrication of growth factor-loaded microspheres within porous nondegradable hydrogels: Controlled release for cartilage tissue engineering.” Journal of Controlled Release 157 (1): 39-45.

K.L. Spiller, S.A. Maher, A.M. Lowman. 2011 “Hydrogels for the Repair of Articular Cartilage Defects.” Tissue Engineering Part B: Reviews 17 (4): 288-299.

J.L. Holloway, K.L. Spiller, A.M. Lowman, G.R. Palmese. 2011 “Analysis of the in vitro swelling behavior of poly(vinyl alcohol) hydrogels in osmotic pressure solution for soft tissue replacement.” Acta Biomaterialia 7 (6): 2477-2482. 

 K.L. Spiller, J.L. Holloway, M.E. Gribb, A.M. Lowman. 2011 “Design of semi-degradable hydrogels based on poly(vinyl alcohol) for cartilage tissue engineering.” Journal of Tissue Engineering and Regenerative Medicine 5 (8): 636-647.

K.L. Spiller, S.J. Laurencin, A.M. Lowman. 2009 “Characterization of the behavior of porous hydrogels in model osmotically-conditioned articular cartilage systems.” Journal of Biomedical Materials Research B 90 (2): 752-759.

K.L. Spiller, S.J. Laurencin, D. Charlton, S. Maher, A.M. Lowman. 2008 “Superporous hydrogels for cartilage repair: Evaluation of the Morphological and Mechanical Properties.” Acta Biomaterialia 4 (1): 17-25.

D. Charlton, M. Peterson, K.L. Spiller, A.M. Lowman, P. Torzilli, S. Maher. 2008 “Semi-Degradable Scaffold for Articular Cartilage Replacement.” Tissue Engineering Part A 14 (1): 207-213.
Click here for the full list of KL Spiller publications at NCBI